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KDN-04C automatic nitrogen determination instrument is an advanced technology for judging the end point of titration by sensors, and the end point is judged with good accuracy, which can reduce the error caused by visual deviation, and the recovery of nitrogen after automatic nitrogen determination after distillation The rate can reach 99.8% to 101%. The KDN-04C automatic nitrogen analyzer measures the protein content of whole wheat and evaluates the relationship between gluten and protein.
Research method: Accurately measure 10ml (NH4)2S04 solution, and use KDN-04C automatic nitrogen analyzer to directly add distilled solution and measure its nitrogen content. Reagents and Instruments: The instrument includes an electronic balance and a KDN-04C automatic nitrogen determination instrument. The reagent is a 0.42% (NH4)2S04 solution. At a high temperature of 105°C, 4.2000 g of the reagent is dried and ground into a powder in (NH4). ) 2S04 powder was added with 1000m1 of water to make a mixed solution.
The recovery rate of the (NH4)2S04 solution directly distilled by the KDN-04C automatic nitrogen analyzer was between 99.8% and 101.0%, which is in accordance with the national standard GB/T5511-2008. By measuring the protein standard can reach the normal range, multiple determination of the average level of 0.9%, KDN-04C automatic nitrogen determination device has better accuracy and precision, for the evaluation of wheat wet gluten is also a feasible Methods.
Determination of protein by automatic nitrogen determination instrument and evaluation of wet gluten
Under normal circumstances, straw feed quality is the worst, protein content is the lowest, and it is also poor. The protein content of grass feed for grasses is relatively low. The protein content of legumes and oil cake feeds is relatively abundant, the quality of plant feeds is poor, and the content is low. , And animal feed quality is better and the content is higher. The content of crude protein in wheat grains is usually between 11.0% and 18.0%, which is an important indicator for evaluating wheat quality. The determination of the protein by the KDN-04C automatic analyzer is now the main method for evaluation.